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Determination of three tanshinones in Salviae miltiorrhizae health tea by HPLC.

Yuanyuan Zhang, Yutao Hu

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Abstract

[Objective] To determine the contents of three major tanshinones in Salviae miltiorrhizae health tea by HPLC.[Method] The HPLC conditions were as follows:Insertsil ODS-SP column(4.6 mm×250 mm,5 μm),mobile phase of acetonitrile-water(V:V=62:38),detection wavelength of 270 nm,flow rate of 1.0 ml/min,sampling amount of 15 μl and column temperature of 30 ℃.[Result] Three compositions of tanshinones were well-separated and the average recovery of cryptotanshinone,tanshinone Ⅰ,tanshinone ⅡA were 98.2%,98.7% and 98.4% respectively(RSD of 1.20%,1.96% and 1.74%,n = 3).The content of the tanshinones in the sample were 1.19,0.79 and 2.25 mg/g respectively.[Conclusion] HPLC has the advantages of simple,rapid and reliable,which can be used to control the quality of Salviae miltiorrhizae health tea.

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What this paper is about

[Objective] To determine the contents of three major tanshinones in Salviae miltiorrhizae health tea by HPLC.[Method] The HPLC conditions were as follows:Insertsil ODS-SP column(4.6 mm×250 mm,5 μm),mobile phase of acetonitrile-water(V:V=62:38),detection wavelength of 270 nm,flow rate of 1.0 ml/min,sampling amount of 15 μl and column temperature of 30 ℃.[Result] Three compositions of tanshinones were well-separated and the average recovery of cryptotanshinone,tanshinone Ⅰ,tanshinone ⅡA were 98.2%,98.7% and 98.4% respectively(RSD of 1.20%,1.96% and 1.74%,n = 3).The content of the tanshinones in the sample were 1.19,0.79 and 2.25 mg/g respectively.[Conclusion] HPLC has the advantages of simple,rapid and reliable,which can be used to control the quality of Salviae miltiorrhizae health tea.

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Available abstract

[Objective] To determine the contents of three major tanshinones in Salviae miltiorrhizae health tea by HPLC.[Method] The HPLC conditions were as follows:Insertsil ODS-SP column(4.6 mm×250 mm,5 μm),mobile phase of acetonitrile-water(V:V=62:38),detection wavelength of 270 nm,flow rate of 1.0 ml/min,sampling amount of 15 μl and column temperature of 30 ℃.[Result] Three compositions of tanshinones were well-separated and the average recovery of cryptotanshinone,tanshinone Ⅰ,tanshinone ⅡA were 98.2%,98.7% and 98.4% respectively(RSD of 1.20%,1.96% and 1.74%,n = 3).The content of the tanshinones in the sample were 1.19,0.79 and 2.25 mg/g respectively.[Conclusion] HPLC has the advantages of simple,rapid and reliable,which can be used to control the quality of Salviae miltiorrhizae health tea.

Key concepts: High-performance liquid chromatography, Chemistry, Chromatography, Acetonitrile

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