Inhibition of Total Phenolics Acid Extracted from Oenanthe Javanica on HBsAg and HBeAg Secretion in Cultured Cell line 2.2.15

Aim To observe the inhibition of total phenolics acid extracted from Oenanthe Javanica(OJTPA) on HBsAg,HBeAg in cultured cell line 2.2.15.Methods 2.2.15 cells were studied as an in vitro model,the maximum non-toxic concentrations(TC0) were identified by cell toxicity experiment of OJTPA before the drug was diluted into 500,250 and 125 μg·mL-1.2.2.15 cells were treated and the medium of the same concentration was replaced every 4 days.On the 8th day the medium was changed to drug-blank medium.The liquid was collected on the 4th,8th day and 4 days after treatment.Results The maximum non-tyxic concentration of OJTPA was 500μg·mL-1.At this concentration,HBeAg was obviously inhibited.On the 8th day the inhibition ratio was 59.33% and 90%;IC50 was 484.17 and 379.89μg·mL-1;TI was 3.158 and 4.025 respectively.HBeAg was superior to HBsAg in terms of inhibition.Conclusion OJTPA can significantly inhibit HBsAg,HBeAg on cultured cell line 2.2.15.