Inhibition of Qinling Chongji on HBsAg and HBeAg secretion in cultured cell line 2215

AIM To study the inhibiting effect of Qinling Chongji ( QL ) on HBsAg and HBeAg in the cultured hepatocarcinoma cell line 2215 (Hep G2) which was genetransfered with hepatitis·B virus (HBV) and its toxicity.METHODS Twenty-four hours after 2215 cells vaccination, the varied concentrations of QL were added to culture fluid, and exchanged the drugs every four days until to 8 d and 12 d. The cell alterations were observed under microscopy in order to evaluate the toxicity of QL. After treated with QL for 8 d and 12 d, the culture fluids were collected and the content of HBeAg and HBsAg was determined by using radioimmunoassay kits. RESULTS QL in nontoxic concentration significantly inhibited the secretion of HBsAg and HBeAg in cell line 2215 after 8 d and 12 d treatment. The mean inhibiting rate of QL (4000 μg·mL-1) on HBeAg was 51.57%±2.14% (8d) and 65.06%±1.94% (12 d), [ IC50 = (8 d) 3090.19 μg·mL-1±772.40 μg·mL-1 and (12 d) 907.13 μg·mL-1±93.57 μg·mL-1]; and was (8 d) 55.04% ±3.88% and (12d) 61.39%±0.98%, (IC50) = (8d) 2993.89 μg·mL-1±768.66 μg·mL-1 and (12 d) 1744.44 μg·mL-1±214.08 μg·mL-1 respectively. CONCLUSION The results show that QL can inhibit the secretion of HBsAg and HBeAg in cell line 2215 and has no toxicity at high concentration.