Content determination of ginsenoside Rg_1,Re and Rd in stems and leaves of Panax ginseng by HPLC-ELSD assay

Objective To establish a HPLC-ELSD method for determining the contents of ginsenoside Rg1,Re and Rd in Panax ginseng. Methods With a Diamonsil C18 column(200 mm×4.6 mm,5 μm),acetonitrile(A)-water(B)was used as the mobile phase at a flow rate of 1.0 ml/min,and eluted in gradient mode(0-30 min,21%A;30-35 min,21%-33%A;35-60 min,33%A;60-65 min,33%-21%A;65-70 min,21%A).An evaporative light-scattering detector(ELSD)was used as the detector with atomization temperature of 35 ℃,evaporation temperature of 50 ℃,and N2 as carrier gas at the pressure of 1.5 Bar. Results The ginsenoside Rg1,Re and Rd had a good linearity in 1.06-7.95 μg,1.94-14.55 μg,1.26-9.45 μg,respectively,and their average recoveries were 96.75%(RSD=1.99%),97.77%(RSD=1.33%),96.30%(RSD=1.26),respectively. Conclusion The method is sensitive,simple and accurate,and can be used for the content determination of ginsenoside.