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Determination of Ginsenoside Rg_1 and Rb_1 in Yushangling Capsule by HPLC-ELSD

Shunliang Zheng

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Abstract

Objective To establish an HPLC-ELSD method for determination of ginsenoside Rg1 and Rb1 in Yushangling capsule. Methods The separation was performed in a Hypersil ODS2 column with a mobile phase of acetonitrile-water in gradient elution,ELSD nebulization at 110 ℃ and flow rate of nitrogen at 3.02~3.05 SLPM.Results The linear range of ginsenoside Rg1 was 2.272~11.360 μg,r = 0.999 1.The linear range of ginsenoside Rb1 was 2.232~11.160 μg,r = 0.999 4.The average recoveries of ginsenoside Rg1 and Rb1 were 96.6 %,98.6 % respectively,and RSD were 1.7 %,2.3%.Conclusion This method is simple,accurate and reliable.It can be used for quality control of Yushangling capsule.

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What this paper is about

Objective To establish an HPLC-ELSD method for determination of ginsenoside Rg1 and Rb1 in Yushangling capsule. Methods The separation was performed in a Hypersil ODS2 column with a mobile phase of acetonitrile-water in gradient elution,ELSD nebulization at 110 ℃ and flow rate of nitrogen at 3.02~3.05 SLPM.Results The linear range of ginsenoside Rg1 was 2.272~11.360 μg,r = 0.999 1.The linear range of ginsenoside Rb1 was 2.232~11.160 μg,r = 0.999 4.The average recoveries of ginsenoside Rg1 and Rb1 were 96.6 %,98.6 % respectively,and RSD were 1.7 %,2.3%.Conclusion This method is simple,accurate and reliable.It can be used for quality control of Yushangling capsule.

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Available abstract

Objective To establish an HPLC-ELSD method for determination of ginsenoside Rg1 and Rb1 in Yushangling capsule. Methods The separation was performed in a Hypersil ODS2 column with a mobile phase of acetonitrile-water in gradient elution,ELSD nebulization at 110 ℃ and flow rate of nitrogen at 3.02~3.05 SLPM.Results The linear range of ginsenoside Rg1 was 2.272~11.360 μg,r = 0.999 1.The linear range of ginsenoside Rb1 was 2.232~11.160 μg,r = 0.999 4.The average recoveries of ginsenoside Rg1 and Rb1 were 96.6 %,98.6 % respectively,and RSD were 1.7 %,2.3%.Conclusion This method is simple,accurate and reliable.It can be used for quality control of Yushangling capsule.

Key concepts: Ginsenoside Rg1, Chromatography, Capsule, Chromatography detector, Chemistry, Gradient elution, High-performance liquid chromatography, Linear range

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