Determination of ginsenoside R_e in total ginsenoside from stalk and leaf of Panax ginseng

Objective: To determine quantitatively ginsenoside Re in total ginsenoside from stalk and leaf of Panax ginseng,and to provide experimental evident for total ginsenoside quality control of Panax ginseng.Methods:The method was developed with the condition as follows:Diamonsil C18(4.6 mm×25 mm,5 μm) and DIKMA EasyGuard C18(10 mm×4.6 mm) was used as chromatographic column and guard column respectively.Gradient elution was employed with the mobile phase of acetonitrile-0.5% acetic acid/water at a flow rate of 1.25 mL/min.ELSD:temperature of the drift tube 40 ℃;gas pressure: 3.4 bar;gain: 7.Results:The standard curve: C=1.191×10-7A-0.187 6;the linear range: 0.038～1.14 mg/mL,r=0.999 3;the limits of detection: 20 ng(S/N3);recovery rate of the added sample: 96.84%～104.21%.Conclusion:With its accuracy,sensitivity,reproducibility as well as the simplicity of sample preparation,this method is feasible for quality control of total ginsenoside from stalk and leaf of Panax ginseng.