[Studies on callus induced from leaves and plantlets regeneration of the traditional Chinese medicine Chrysanthemum morifolium].

OBJECTIVE: To set up the optimums for the leaf tissue culture of Chrysanthemum morifolium cultivated in Anhui Province. METHOD: Leaves were cut and cultured onto the same kind of media on different sides and defferent kinds of media on the same side, and induced to form the whole plantlet. RESULT AND CONCLUSION: All the media used could induce callus, while the results of callus redifferentiation were very distinct. The media with NAA 0.1 mg.L-1 + 6-BA 0.1-1.0 mg.L-1 were much better than others when callus were induced. The MS medium with 6-BA 2.0 mg.L-1 and NAA 0.5 mg.L-1 was the preferable medium for the bud sprouting. The regenerated plantlets produced variation of leaf shape.