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2006•Lishizhen Medicine and Materia Medica ResearchRequires access

Determination of Magnolol and Honokiol in Compound Houpu Granule By HPLC

Tan Ling Ling

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Abstract

ObjectiveTo establish an HPLC method for the determination of magnolol and honokiol in Compound Houpu Granule.MethodsThe samples were analyzed on a Diamondsil~(CM)C_(18) column(6.4 mm×200 mm,5μm).The mobile phase was a mixture of MeOH-0.1%H_3PO_4(78:22);the rate of flow was 1ml/min;the column temperature was 35 ℃;the detection wavelength was 294 nm.ResultsCalibration graph was linear in the range of 0.041 1~0.822 2 μg,r=0.999 9 for magnolol,(0.023 06)~0.456 12μg,r=1 for honokiol.The recovery was 97.1%,RSD=1.27% for magnolol;96.7%,RSD=1.25% for honokiol.ConclusionThe method is sensitive and its results are accurate with good reproducibility.It can be used for the quality control of Compound Houpu Granule.

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ObjectiveTo establish an HPLC method for the determination of magnolol and honokiol in Compound Houpu Granule.MethodsThe samples were analyzed on a Diamondsil~(CM)C_(18) column(6.4 mm×200 mm,5μm).The mobile phase was a mixture of MeOH-0.1%H_3PO_4(78:22);the rate of flow was 1ml/min;the column temperature was 35 ℃;the detection wavelength was 294 nm.ResultsCalibration graph was linear in the range of 0.041 1~0.822 2 μg,r=0.999 9 for magnolol,(0.023 06)~0.456 12μg,r=1 for honokiol.The recovery was 97.1%,RSD=1.27% for magnolol;96.7%,RSD=1.25% for honokiol.ConclusionThe method is sensitive and its results are accurate with good reproducibility.It can be used for the quality control of Compound Houpu Granule.

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Available abstract

ObjectiveTo establish an HPLC method for the determination of magnolol and honokiol in Compound Houpu Granule.MethodsThe samples were analyzed on a Diamondsil~(CM)C_(18) column(6.4 mm×200 mm,5μm).The mobile phase was a mixture of MeOH-0.1%H_3PO_4(78:22);the rate of flow was 1ml/min;the column temperature was 35 ℃;the detection wavelength was 294 nm.ResultsCalibration graph was linear in the range of 0.041 1~0.822 2 μg,r=0.999 9 for magnolol,(0.023 06)~0.456 12μg,r=1 for honokiol.The recovery was 97.1%,RSD=1.27% for magnolol;96.7%,RSD=1.25% for honokiol.ConclusionThe method is sensitive and its results are accurate with good reproducibility.It can be used for the quality control of Compound Houpu Granule.

Key concepts: Magnolol, Honokiol, Granule (geology), Chromatography, Chemistry, High-performance liquid chromatography, Reproducibility, Linear range

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