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2002•Pharmaceutical Journal of Chinese People's Liberation ArmyRequires access

Determination of evodiamine and rutaecarpine In Wuji Wan by HPLC

Tan Sheng

Open publisher page 1 citations

Abstract

Aim To develop a quantitative method for the determination of evodiamine and rutaecarpine in Wuji Wan by HPLC.Method The chromatographic conditions include column Intersil C 18 ,mobile phase:acetonitrile-10% acetonitrile(50:50),flow rate 1ml/min,wavelength 225nm.Results The number of theoretical plates calculated by evodiamine and rutaecarpine peak were 2 681 and 2 067 respectively.The regression equation of evodiamine was Y=0.076 46+0.000 000 196 5X (r=0.999 9) and the linear range was 10.2～51.0μg·ml -1 .The regression equation of rutaecarpine was Y=-0.219 9+0.000 000 365 8X (r=0.999 9) and the linear range was 10.0～50.0μg·ml -1 .The average recovery of evodiamine was 97.3% (n=5),RSD 3.2%;The average recovery of rutaecarpine was 101.4% (n=5),RSD 3.9%.The measurable lowest limit of evodiamine and rutaecarpine were 0.05 and 0.1μg·ml -1 respectively.Conclusion The method was sensitive,rapid and accurate.

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What this paper is about

Aim To develop a quantitative method for the determination of evodiamine and rutaecarpine in Wuji Wan by HPLC.Method The chromatographic conditions include column Intersil C 18 ,mobile phase:acetonitrile-10% acetonitrile(50:50),flow rate 1ml/min,wavelength 225nm.Results The number of theoretical plates calculated by evodiamine and rutaecarpine peak were 2 681 and 2 067 respectively.The regression equation of evodiamine was Y=0.076 46+0.000 000 196 5X (r=0.999 9) and the linear range was 10.2～51.0μg·ml -1 .The regression equation of rutaecarpine was Y=-0.219 9+0.000 000 365 8X (r=0.999 9) and the linear range was 10.0～50.0μg·ml -1 .The average recovery of evodiamine was 97.3% (n=5),RSD 3.2%;The average recovery of rutaecarpine was 101.4% (n=5),RSD 3.9%.The measurable lowest limit of evodiamine and rutaecarpine were 0.05 and 0.1μg·ml -1 respectively.Conclusion The method was sensitive,rapid and accurate.

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Available abstract

Aim To develop a quantitative method for the determination of evodiamine and rutaecarpine in Wuji Wan by HPLC.Method The chromatographic conditions include column Intersil C 18 ,mobile phase:acetonitrile-10% acetonitrile(50:50),flow rate 1ml/min,wavelength 225nm.Results The number of theoretical plates calculated by evodiamine and rutaecarpine peak were 2 681 and 2 067 respectively.The regression equation of evodiamine was Y=0.076 46+0.000 000 196 5X (r=0.999 9) and the linear range was 10.2～51.0μg·ml -1 .The regression equation of rutaecarpine was Y=-0.219 9+0.000 000 365 8X (r=0.999 9) and the linear range was 10.0～50.0μg·ml -1 .The average recovery of evodiamine was 97.3% (n=5),RSD 3.2%;The average recovery of rutaecarpine was 101.4% (n=5),RSD 3.9%.The measurable lowest limit of evodiamine and rutaecarpine were 0.05 and 0.1μg·ml -1 respectively.Conclusion The method was sensitive,rapid and accurate.

Key concepts: Evodiamine, Rutaecarpine, Chromatography, Chemistry, High-performance liquid chromatography, Linear range, Detection limit

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