Improvement the method of type 1 diabetic rat model induced by Streptozotocin

Objective To compare the success rate of taking food and fasting before induce type l diabetic rat by intraperitoneal injection Streptozotocin(50 mg/kg).Determine survival rate after two weeks.Then those model rats were applied pancreas islets transplantation.Methods 40 Wistar rats were random divided into 2 groups.The first group taking food freely before intraperitoneal inject Streptozotocin and the second group is fasting for 12 hours then intraperitoneal inject Streptozotocin.A successful model is determine by two times random blood suger level higher than 16.8 mmol/L and occurrence typical diabetes symptom for two weeks.Then determine model survival rate.Execution operation of pancreas islets transplantation.Results The first group success rate is 100%.The second group success rate is 75%.The statistics difference of success rate between two methods is less than 0.05.All model rats survived post-operation.Blood suger could reach normal level.Correlation circs need observation further.Conclusion It is a high success rate and stable method intraperitoneal injection STZ inducing diabetic rat without fasting.Model rat has good tolerance to the wound of operation.