2011•Yaowu fenxi zazhiRequires access

HPLC determination of icariin content in Zhuanggu Zhitong granule

Ying Gao

Abstract

Objective:To determine icariin content in Zhuanggu Zhitong granule by HPLC.Methods:Thermo C18 column(250 mm×4.6 mm,5 μm)was used.Acetonitrile-water(26∶74)was used as themobile phase,the flow rate was 1.0 mL·min-1,the detected wavelength was 270 nm,the temperature of column was controlled at room temperature.Results:The calibration curve was linear in the range of 0.01-0.07 mg·mL-1 for icariin(r=0.9999).The average recovery was 99.5%(n=6),RSD was 0.85%.Conclusion:The method is simple,accurate and reliable.

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Key concepts: Icariin, Chemistry, Chromatography, Granule (geology), Calibration curve, High-performance liquid chromatography, Content determination, Analytical Chemistry (journal)