Screening and analysis of metallo-β-lactamase -producting Pseudomonas aeruginosa in the burn ward

Objective To compare the resistance of metallo-β-lactamase-producting Pseudomonas aeruginosa between burn ward and other wards,so as to provide basis for reasonable use of antimicrobial agents.Methods BD Phoenix-100 bacteria identification and drug sensitivity analyzer was used to detect Pseudomonas aeruginosa and its susceptibility,and disc synergy method was used for the screening of metallo-β-lactamase-producting Pseudomonas aeruginosa.Results Of 68 strains of Pseudomonas aeruginosa,9 strains(13.2%) were metallo-β-lactamase(MBL) positive.Among the 9 metallo-β-lactamase positive strains,7 strains were from the burn ward and 2 strains from other wards.The resistance rate of the MBL-producing strains to ceftazidime,cefotaxime,imipenem,ciprofloxacin, amikacin,gentamicin,piperacillin and aztreonam was respectively 100.0%,100.0%,88. 9%,66.7%,88.9%,100,0%,100.0%,and 88.9%,obviously higher than that of non-MBL-producing Pseudomonas aeruginosa.Conclusion The rate of MBL-producing Pseudomonas aeruginosa is obviously higher in burn ward than that in other wards.The resistance of MBL-producing Pseudomonas aeruginosa is high against commonly used antimicrobial agents.Clinical use of antibiotics should be based on the antimicrobial susceptibility test results.