Metallo-β-lactamase Detection and Antimicrobial Resistance Analysis of Pseudomonas Aeruginosa Causing Nosocomial Infection

Objective:To study the metallo-β-lactamase production and antimicrobial resistance of Pseudomonas aeruginosa causing nosocomial infection for reasonable use of antimicrobial agents.Methods:The microbiolgy sensitivity synergic tests were processed by K-B method on the MH agar to detect metallo-β-lactamase and antimicrobial resistance were tested by use of rapid identification and susceptibility testing system with the VITEK2 system(bioMerieux).Results:67(14.7%) imipenem resistant strains were identified from 456 strains of Pseudomonas aeruginosa causing nosocomial infection.Among 67 imipenem resistant strains,24(5.3%) strains produced Metallo-β-lactamase resistant to cefotaxime,piperacillin,ciprofloxacin,amikacin,ceftazidime,cefepime,cefoperazone/sulbactam and aztreonam,the resistance rate of the strains producing Metallo-β-lactamase that were 95.8%,95.8%,91.7%,91.7%,79.2%,71.0%,58.3% and 54.2% respectively,obviously higher than that of the strains notproducing Metallo-β-lactamase.Conclusion:Among the strains of Pseudomonas aeruginosa causing nosocomial infection,to clinical common antimicrobial agents the resistance rate of the strains producing metallo-β-lactamase were obviously more than the strains notproducing Metallo-β-lactamase.