2008•Research and Practice on Chinese MedicinesRequires access

Determination of Chlorogenic Acid and Baicalin in Yinhuang Granules by HPLC

Ji Ma

Abstract

Objective To develop an method for simultaneous determination of chlorogenic acid and baicalin in Yinhuang granules.Methods The chromatographic analysis were carried out on a HYPERSIL Clscohmn(4.6 mm ×150 mm,5 μm) with methanol-0.2% phosphoric acid as mobile phase and 0.8 mL·min-1 flow rate in gradient mode.Results The linear ranges of chlomgenic acid and baicalin were from 0.23 μg to 1.14 μg(r=0.999 9) and from 0.33 μg to 1.64 μg(r=0.999 9),the mean recoveries of chlomgenic acid and baicalin were 100.12%with RSD of 0.84% and 102.4% with RSD of 1.47%respectively.Conclusion The method is simple,fast and accurate.It can be used to control for the quality of Yinhuang granules.

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Key concepts: Baicalin, Chlorogenic acid, Chromatography, Phosphoric acid, Chemistry, High-performance liquid chromatography, Organic chemistry