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2004•Research and Practice on Chinese MedicinesRequires access

Determination of Chlorogenic Acid and Baicalin in Yinhuang Granules by HPLC

Yuqing Li

Open publisher page 2 citations

Abstract

Objective To determine the content of chlorogenic acid and baicalin in Yinhuang Granules.Methods HPLC was used with ODS C_(18) column.Acetonitrile-1%acetic acid(1∶9)and methanol-0.1%orthophosphoric acid were used as mobile phases.The detection wavelength was set at 326 nm and 274 nm.Results The sample was easy to deal with and the chromatogram's separability was good with a good reproducibility.Conclusion These methods can be used to control the quality of YinhuangGranules.

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What this paper is about

Objective To determine the content of chlorogenic acid and baicalin in Yinhuang Granules.Methods HPLC was used with ODS C_(18) column.Acetonitrile-1%acetic acid(1∶9)and methanol-0.1%orthophosphoric acid were used as mobile phases.The detection wavelength was set at 326 nm and 274 nm.Results The sample was easy to deal with and the chromatogram's separability was good with a good reproducibility.Conclusion These methods can be used to control the quality of YinhuangGranules.

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Available abstract

Objective To determine the content of chlorogenic acid and baicalin in Yinhuang Granules.Methods HPLC was used with ODS C_(18) column.Acetonitrile-1%acetic acid(1∶9)and methanol-0.1%orthophosphoric acid were used as mobile phases.The detection wavelength was set at 326 nm and 274 nm.Results The sample was easy to deal with and the chromatogram's separability was good with a good reproducibility.Conclusion These methods can be used to control the quality of YinhuangGranules.

Key concepts: Baicalin, Chlorogenic acid, Chromatography, High-performance liquid chromatography, Chemistry, Acetic acid, Acetonitrile, Reproducibility

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