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Determination of danshensu sodium in Danshen Shuxin Capsule by HPLC

Long Meng-lin

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Abstract

OBJECTIVE To establish an HPLC method for determination of danshensu sodium in Danshen Shuxin Capsule.METHODS Danshensu sodium in Danshen Shuxin Capsule was determined by HPLC,using Kromasil C18 column,acetonitrile-1.2% acetic acid(9∶91) as mobile phase,the detection wavelength was 280nm.The flow rate was 1.0mL·min-1.RESULTS The linear range of danshensu sodium was 23.2～232μg·mL-1,coefficient correlation r was 0.9997,the average recovery was 98.96 %,RSD=1.59%.CONCLUSION The method was simple,rapid,accurate and repreatable.It can be used for determination of danshensu sodium in Danshen Shuxin Capsule.

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OBJECTIVE To establish an HPLC method for determination of danshensu sodium in Danshen Shuxin Capsule.METHODS Danshensu sodium in Danshen Shuxin Capsule was determined by HPLC,using Kromasil C18 column,acetonitrile-1.2% acetic acid(9∶91) as mobile phase,the detection wavelength was 280nm.The flow rate was 1.0mL·min-1.RESULTS The linear range of danshensu sodium was 23.2～232μg·mL-1,coefficient correlation r was 0.9997,the average recovery was 98.96 %,RSD=1.59%.CONCLUSION The method was simple,rapid,accurate and repreatable.It can be used for determination of danshensu sodium in Danshen Shuxin Capsule.

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Available abstract

OBJECTIVE To establish an HPLC method for determination of danshensu sodium in Danshen Shuxin Capsule.METHODS Danshensu sodium in Danshen Shuxin Capsule was determined by HPLC,using Kromasil C18 column,acetonitrile-1.2% acetic acid(9∶91) as mobile phase,the detection wavelength was 280nm.The flow rate was 1.0mL·min-1.RESULTS The linear range of danshensu sodium was 23.2～232μg·mL-1,coefficient correlation r was 0.9997,the average recovery was 98.96 %,RSD=1.59%.CONCLUSION The method was simple,rapid,accurate and repreatable.It can be used for determination of danshensu sodium in Danshen Shuxin Capsule.

Key concepts: Chromatography, Chemistry, Capsule, High-performance liquid chromatography, Sodium, Acetic acid, Biochemistry, Organic chemistry

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