2006•Journal of Chengdu UniversityRequires access

Determination of Danshensu in Compound Danshen Capsules by RP-HPLC

Zou Liang, Luo Ya-ling

Abstract

To determine the content of Danshensu in Compound Danshen Capsules,RP-HPLC method is used.The samples are separated on Dikma HPLC column with the mobile phrase of acetonitrile-water-glacial acetic(5∶160∶1).The flow-rate is 1.0mL:min1 and the wavelength is 280nm with column temperature of 30℃.The linear calibration curves are obtained at the range of 0.28~1.40μg(r=0.9998).The average recovery of Danshensu is 100.17%(RSD=0.78%,n=6) in the capsules.The method is precise(RSD=0.64%).The method is simple,quick and accurate,and proves suitable for determining the content of Danshensu in Compound Danshen Capsules.

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Key concepts: Chromatography, High-performance liquid chromatography, Content determination, Chemistry, Calibration curve, Acetonitrile, Analytical Chemistry (journal), Detection limit