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2001•Chinese Journal of Hospital PharmacyRequires access

Determination of baicalin in Pizhenqing granules with HPLC

Lu Jing

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Abstract

OBJECTIVE To establish a method for determining the content of baicalin in Pizhenqing granules by HPLC.METHODS A nucleosil C 18 column with acetonitrile 1% acetic acid solution (26∶74) as the mobile phase was used.The flow rate was 1.2 ml·min -1 and the detective wavelength was 276 nm.RESULTS The calibration curve was linear at the range of 15～41 μg for baicalin ( r = 0.9994 ).The average recovery was 99.16% and RSD was 0.92% ( n =6).CONCLUSIONS The method is simple,and accurate and can be used as quantitative method for this preparation.

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OBJECTIVE To establish a method for determining the content of baicalin in Pizhenqing granules by HPLC.METHODS A nucleosil C 18 column with acetonitrile 1% acetic acid solution (26∶74) as the mobile phase was used.The flow rate was 1.2 ml·min -1 and the detective wavelength was 276 nm.RESULTS The calibration curve was linear at the range of 15～41 μg for baicalin ( r = 0.9994 ).The average recovery was 99.16% and RSD was 0.92% ( n =6).CONCLUSIONS The method is simple,and accurate and can be used as quantitative method for this preparation.

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Available abstract

OBJECTIVE To establish a method for determining the content of baicalin in Pizhenqing granules by HPLC.METHODS A nucleosil C 18 column with acetonitrile 1% acetic acid solution (26∶74) as the mobile phase was used.The flow rate was 1.2 ml·min -1 and the detective wavelength was 276 nm.RESULTS The calibration curve was linear at the range of 15～41 μg for baicalin ( r = 0.9994 ).The average recovery was 99.16% and RSD was 0.92% ( n =6).CONCLUSIONS The method is simple,and accurate and can be used as quantitative method for this preparation.

Key concepts: Baicalin, Chromatography, Calibration curve, High-performance liquid chromatography, Chemistry, Acetic acid, Acetonitrile, Linear range

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