ScholarLens Back to search
2005•Journal of Military Surgeon in Southwest ChinaRequires access

Determination of Baicalin in Xincang Granules by HPLC

Zeng Ren-jie

Open publisher page 0 citations

Abstract

Objective To establish a method for the determination of baicalin content in Xincang Granules.Methods HPLC was adopted with C_~18 column (250mm×4.6mm,5μm);The mobile phase was methanol-water (adapted pH 3.0 by phosphoric acid)(44∶56).The detective wavelength was at 278nm,the flow rate was 1.0ml·min~-1 .Results The calibration curve was linear in the range of 2.0～25.0μg·ml~-1 (r=0.9996);The average recovery was 97.89%,and RSD was 0.98%.Conclusions The method is simple,rapid and accurate.

About this research paper

What this paper is about

Objective To establish a method for the determination of baicalin content in Xincang Granules.Methods HPLC was adopted with C_~18 column (250mm×4.6mm,5μm);The mobile phase was methanol-water (adapted pH 3.0 by phosphoric acid)(44∶56).The detective wavelength was at 278nm,the flow rate was 1.0ml·min~-1 .Results The calibration curve was linear in the range of 2.0～25.0μg·ml~-1 (r=0.9996);The average recovery was 97.89%,and RSD was 0.98%.Conclusions The method is simple,rapid and accurate.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To establish a method for the determination of baicalin content in Xincang Granules.Methods HPLC was adopted with C_~18 column (250mm×4.6mm,5μm);The mobile phase was methanol-water (adapted pH 3.0 by phosphoric acid)(44∶56).The detective wavelength was at 278nm,the flow rate was 1.0ml·min~-1 .Results The calibration curve was linear in the range of 2.0～25.0μg·ml~-1 (r=0.9996);The average recovery was 97.89%,and RSD was 0.98%.Conclusions The method is simple,rapid and accurate.

Key concepts: Baicalin, Chromatography, Phosphoric acid, Medicine, Calibration curve, High-performance liquid chromatography, Linear relationship, Content determination

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of Baicalin in Xincang Granules by HPLC — Research Paper | ScholarLens