HPLC determination of icariin in Shenbao capsules
Ling Li
Abstract
Ling Li
Abstract
Objective:To establish an HPLC method for determination of icariin in Shenbao capsules.Methods: The Bakman ODS C_(18)(4.6mm×250mm,5μm)was used with a mobile phase consisting of acetonitrile-water(30 :70)by flow rate 1.0mL·min~(-1) in this method.And the detection was set at 270nm.Results:The linear range of icariin was 0.1375-2.20μg(r=0.9999).The average recovery rate was 98.9%(n=5).Conclusion:The meth- od is rapid,simple and precise.
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Objective:To establish an HPLC method for determination of icariin in Shenbao capsules.Methods: The Bakman ODS C_(18)(4.6mm×250mm,5μm)was used with a mobile phase consisting of acetonitrile-water(30 :70)by flow rate 1.0mL·min~(-1) in this method.And the detection was set at 270nm.Results:The linear range of icariin was 0.1375-2.20μg(r=0.9999).The average recovery rate was 98.9%(n=5).Conclusion:The meth- od is rapid,simple and precise.
Key concepts: Icariin, Chemistry, Chromatography, High-performance liquid chromatography, Linear range, Linear relationship, Recovery rate, Volumetric flow rate