2005•Huaxi yaoxue zazhiRequires access

Determination of icariin content in Gusongbao granule by HPLC

Zhifeng Zhang

Abstract

OBJECTIVE To determine icariin content in Gusongbao granule. METHODS HPLC method was performed on a C_(18) column.The mobile phase consisted of acetonitrile-water(2674) and the UV detective wavelength was set at 270 nm. RESULTS The calibration curve of icariin was linear within 0.0331-0.331 mgml~(-1), Y=1.85710~3X+11.180(r=0.9999).CONCLUSION This method is simple,rapid and accurate. It is suitable for the determination of icariin content.

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Key concepts: Icariin, Chemistry, Chromatography, High-performance liquid chromatography, Granule (geology), Calibration curve, Content determination, Analytical Chemistry (journal)