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Study on the quality control standard of Huxin capsules

Deng Shao-wei

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Abstract

Objective:To establish the quality control standard of Huxin capsules.Method:Fructus Evodiae,Rhi- zoma Acori Tatarinowii,Borneolum Syntheticum,and Radix et Rhizoma Notoginseng were identified by TLC.The content of icariin in Huxin capsules was determined by HPLC.Alltima C_(18) column(250 mm×4.6 mm,5 μm) was used.The mobile phase was a mixture of acetonitrile-water-glacial acetic acid(27:73:0.5),column temperature at 30 ℃,the flow rate was 1 mL·min~(-1) and the detection wavelength was at 270 nm.Results:The calibration curve was linear in the range of 0.1-3.1 μg for icariin (r=0.9999,n=8).The mean recovery of icariin was 100.5%,RSD=1.2%.Conclusion:This method is simple,reliable,accurate,and used to control the quality of Huxin capsules.

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Objective:To establish the quality control standard of Huxin capsules.Method:Fructus Evodiae,Rhi- zoma Acori Tatarinowii,Borneolum Syntheticum,and Radix et Rhizoma Notoginseng were identified by TLC.The content of icariin in Huxin capsules was determined by HPLC.Alltima C_(18) column(250 mm×4.6 mm,5 μm) was used.The mobile phase was a mixture of acetonitrile-water-glacial acetic acid(27:73:0.5),column temperature at 30 ℃,the flow rate was 1 mL·min~(-1) and the detection wavelength was at 270 nm.Results:The calibration curve was linear in the range of 0.1-3.1 μg for icariin (r=0.9999,n=8).The mean recovery of icariin was 100.5%,RSD=1.2%.Conclusion:This method is simple,reliable,accurate,and used to control the quality of Huxin capsules.

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Available abstract

Objective:To establish the quality control standard of Huxin capsules.Method:Fructus Evodiae,Rhi- zoma Acori Tatarinowii,Borneolum Syntheticum,and Radix et Rhizoma Notoginseng were identified by TLC.The content of icariin in Huxin capsules was determined by HPLC.Alltima C_(18) column(250 mm×4.6 mm,5 μm) was used.The mobile phase was a mixture of acetonitrile-water-glacial acetic acid(27:73:0.5),column temperature at 30 ℃,the flow rate was 1 mL·min~(-1) and the detection wavelength was at 270 nm.Results:The calibration curve was linear in the range of 0.1-3.1 μg for icariin (r=0.9999,n=8).The mean recovery of icariin was 100.5%,RSD=1.2%.Conclusion:This method is simple,reliable,accurate,and used to control the quality of Huxin capsules.

Key concepts: Icariin, Chemistry, Chromatography, Calibration curve, High-performance liquid chromatography, Acetic acid, Panax notoginseng, Quality standard

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