Studies on purification process of total alkaloids from Coptis chinensis by macroporous adsorption resin

Objective To investigate the process of isolation and purification of total alkaloids from Coptis chinensis by macroporous adsorption resin.Methods The absorption amount and desorption ratio were taken as the investigation index to evaluated 9 kinds of resins.And the process and parameters of purification of total alkaloids from Coptis chinensis by optimized macroporous adsorption resin were studied.Results The dynamic adsorption and isolation effect of D141 type macroporous adsorption resin was the best,with the drug solution concentration as 30 mg of medicinal material/mL,ratio of diameter and height as 1∶8,velocity of absorption as 2 BV/h,solvent of removing impurities as water,volume of removing impurities as 2 BV,velocity of removing impurities as 1 BV/h,solvent of elution as 50% of ethanol,volume of elution as 4 BV,velocity of elution as 3 BV/h.The purity of total alkaloids in the final product was 67.71% after isolation and purification by macroporous adsorption resin.Conclusion The process is rational,feasible and suitable for industrial production.