2004•Techan yanjiuRequires access

Determination of Icariin in Naogongyang Granule by RP-HPLC

Jiao Lian-qing

Abstract

Objective:A determination method of Icariin in Naogongyang Granule was established. Method: The RP-HPLC was carried out on C_(18) column using methanol-water-acetic acid (35∶25∶0.3)as a mobile phase,the detecting wavelength was set at 270nm,the flow rate was 1.0mL·min~(-1),the column temperature was at 25℃. Result: The linear relationship was good,the average recovery was 98.84% and RSD was 1.83%( n =5). Conclusion: The method is simple,reliable and suitable for the quality control of Naogongyang Granule.

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Key concepts: Icariin, Granule (geology), Chromatography, Chemistry, High-performance liquid chromatography, Acetic acid, Linear relationship, Mathematics