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2009•Guiding Journal of Traditional Chinese Medicine and PharmacyRequires access

Determination of Kakkonein in Ganmaoqingre Granule With HPLC Method

Tang Han-hua

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Abstract

Objective:To establish the determination method of kakkonein in Ganmaoqingre granule.Methods:HPLC method was used,the chromatographic condition consisted of C18 column with methanol-water(22:78) as mobile phase at the detection wavelength of 250 nm.Results:Kakkonein showed a linear relationship at the range of 0.1384-1.384 μg,the average recovery was 99.84%,RSD%=1.65%(n=6).Conclusion:The method is simple,accurate,high sensitivity and good reproducibility,can be used as quality control for Ganmaoqingre granule.

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Objective:To establish the determination method of kakkonein in Ganmaoqingre granule.Methods:HPLC method was used,the chromatographic condition consisted of C18 column with methanol-water(22:78) as mobile phase at the detection wavelength of 250 nm.Results:Kakkonein showed a linear relationship at the range of 0.1384-1.384 μg,the average recovery was 99.84%,RSD%=1.65%(n=6).Conclusion:The method is simple,accurate,high sensitivity and good reproducibility,can be used as quality control for Ganmaoqingre granule.

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Available abstract

Objective:To establish the determination method of kakkonein in Ganmaoqingre granule.Methods:HPLC method was used,the chromatographic condition consisted of C18 column with methanol-water(22:78) as mobile phase at the detection wavelength of 250 nm.Results:Kakkonein showed a linear relationship at the range of 0.1384-1.384 μg,the average recovery was 99.84%,RSD%=1.65%(n=6).Conclusion:The method is simple,accurate,high sensitivity and good reproducibility,can be used as quality control for Ganmaoqingre granule.

Key concepts: Chromatography, Granule (geology), Reproducibility, High-performance liquid chromatography, Chemistry, Linear range, Linear relationship, Chromatographic separation

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