Study on the Purification Process of the Total Flavonoids and Total Saponins of the Tea Root by Macroporous Resin AB-8

Objective To discuss purification technology conditions of total flavonoids and total saponins from tea root by AB-8 macroporous resin.Methods With absorption rate and metastasis rate of total flavonoids and total saponins as indexes,investigated concentration of sample liquid, p H, ratio of macroporous resin to crude drug, concentration of eluted ethanol and other factors. Results Optimum purification conditions were as follows: Concentration of sample liquid was 0.3 g · ml-1, p H of sample liquid was 6,ratio of crude drug to macroporous resin was1 : 2, total flavonoids and total saponins were eluted by 12 BV water,10 BV 30% ethanol and 10 BV 70% ethanol respectively. Conclusion Macroporous resin AB-8 could be used to purify total flavonoids and total saponins from tea root,the content of total flavonoid and total saponins reached more than 60%.