Study on purification of kudzu flavonoids and saponins with macroporous resin

Objective To investigate the simultaneous purification of flavonoids and saponins from puerariae lobatae radix by macroporous resins,for development and industrial production of compound drug containing puerariae lobatae radix. Methods Macroporous resin was chosen with static and dynamic adsorptiion and desorption experiments to optimize the purification parameters. The contents of total flavonoids and total sapoins was taken as the index for investigation. Results D101 macroporous resin was found to have good adsorption and desorption effects.The dynamic adsorption ratio of flavonoids was 54.93 mg/g,After eluted with 4BV of distilled water and 4BV of 30%,50%,70% ethanol respectively. The contents of flavonoids and saponins were 78.89%,36.32% respectively. Conclusion D101 is a ideal resin with the best enrichment for separating and purifying total flavanoids and total saponins from puerariae lobatae radix.