EXPERIMENTAL STUDY ON DISINFECTION PERFORMANCE OF A KIND OF QUICK-DRYING ANTISEPTIC SOLUTION

Objective To study the disinfection performance and influential factors of a kind of quick-drying liquid skin antiseptic with chlorhexidine acetate as the active principle.Methods Suspension quantitative germicidal test and animal toxicity test were used to carry out laboratory observation of this antiseptic.Results At room temperature,the killing rate of Staphylococcus aureus,Escherichia coli,Pseudomonas aeruginosa and Candida albicans exposed to the 10-fold dilution of compound chlorhexidine alcohol antiseptic solution containing chlorhexidine acetate 8 mg/L and 70%(v/v) ethanol for 2 min were 100%.Adding BSA to the bacterial suspension slightly influenced the germicidal efficacy of the antiseptic solution in killing E.coli.With the increase of pH and temperature,the germicidal efficacy of the 8-fold dilution of the antiseptic solution could obviously be enhanced in killing E.coli.The acute oral toxicity test of the disinfection solution showed that LD50 values in both rats were 5 000 mg/kg·bw,which were practically non-toxic.The skin irritation index of stock solution on rabbits was 0.The mouse bone marrow micronucleus test showed no non-nuclear-induced effect.Conclusion The quick-drying antiseptic has rapid germicidal action on vegetative forms of bacteria and natural bacteria on skin,no action of inducing micronucleus,no irritation to skin,and belongs to non-toxic substance in animal test.