EXPERIMENTAL OBSERVATION ON GERMICIDAL EFFICACY AND TOXICITY OF A HAND ANTISEPTIC GEL

Objective To observe the germicidal efficacy and toxicity of a hand antiseptic gel.Methods Carrier quantitative germicidal test and animal toxicity test were used to carry out experimental observation.Results The main germicidal ingredients of the hand antiseptic gel contained 60% ethanol and 10 g/L chlorhexidine gluconate.The killing logarithm values of Escherichia coli,Staphylococcus aureus and Pseudomonas aeruginosa on carriers exposed to this hand antiseptic gel for 0.5 min were 5.00 and that of Candida albicans exposed for 0.5 min was 4.00.The average killing logarithm value of the natural bacteria on hand of volunteers exposed to this hand antiseptic gel for 1 min was1.00.The LD50 of mice acute oral toxicity was ﹥5 000 mg/kg·bw with the disinfection solution.Its cumulative score in skin irritation test by repeated contact with intact skin was 0.The mouse bone marrow polychromatic erythrocytes micronucleus test was negative.Conclusion The hand antiseptic gel has good germicidal efficacy.It belongs to practically non-toxic class.It has no irritation to intact skin and has no mutagenic effect.