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2014•Zhongguo shiyan fangjixue zazhiRequires access

Determination of Concentration of Total Saponins and Oleanolic Acid of Rubus sachalinensis at Different Collecting Time

Wang Bao-zhe

Open publisher page 2 citations

Abstract

Objective: To establish the content determination method for the total saponinsandoleanolic acid of Rubus sachalinensis and the best collection stage. Method: Using the UV-visible spectrophotometry determination the content of total saponins at wavelength of 547 nm and using 5% vanillin-acetic acid and perchloric acid coloration. An HPLC method for oleanolic acid of R. sachalinensis was established to determineoleanolic acid. Result: There were differences in the content of the total saponinsand oleanolic acid of R.sachalinensis at different collecting time; the content of total saponins increased from June to September and reached the highest level in September. However,it gradually decreased in October. Conclusion: The method was simple,rapid,repeatableand could be used for the total saponins and oleanolic acid determination of R.sachalinensis,the best collection stage was September.

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What this paper is about

Objective: To establish the content determination method for the total saponinsandoleanolic acid of Rubus sachalinensis and the best collection stage. Method: Using the UV-visible spectrophotometry determination the content of total saponins at wavelength of 547 nm and using 5% vanillin-acetic acid and perchloric acid coloration. An HPLC method for oleanolic acid of R. sachalinensis was established to determineoleanolic acid. Result: There were differences in the content of the total saponinsand oleanolic acid of R.sachalinensis at different collecting time; the content of total saponins increased from June to September and reached the highest level in September. However,it gradually decreased in October. Conclusion: The method was simple,rapid,repeatableand could be used for the total saponins and oleanolic acid determination of R.sachalinensis,the best collection stage was September.

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Available abstract

Objective: To establish the content determination method for the total saponinsandoleanolic acid of Rubus sachalinensis and the best collection stage. Method: Using the UV-visible spectrophotometry determination the content of total saponins at wavelength of 547 nm and using 5% vanillin-acetic acid and perchloric acid coloration. An HPLC method for oleanolic acid of R. sachalinensis was established to determineoleanolic acid. Result: There were differences in the content of the total saponinsand oleanolic acid of R.sachalinensis at different collecting time; the content of total saponins increased from June to September and reached the highest level in September. However,it gradually decreased in October. Conclusion: The method was simple,rapid,repeatableand could be used for the total saponins and oleanolic acid determination of R.sachalinensis,the best collection stage was September.

Key concepts: Oleanolic acid, Chemistry, Acetic acid, Chromatography, Content determination, High-performance liquid chromatography, Biochemistry, Medicine

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Determination of Concentration of Total Saponins and Oleanolic Acid of Rubus sachalinensis at Different Collecting Time — Research Paper | ScholarLens