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Content determination of Total Saponins from Rubus parvifolius L

Lingyan Ren

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Abstract

Objective; To establish a method for content determination of total saponins in Rubus parvifolius L.. Methods; Total saponins was determined by UV - VIS spectrophotometry after different extracts of the sample had been coloured. Results; The method was linear in the range of 22-176μg (r = 0.9997),and the average recovery was 102. 1 % , RSDwas 2. 82% (n = 6). The content of total saponins was highest by the method of 70% ethanol reflux. Conclusion; The sample is stable after coloured. The method is sensitive and reliable , which may be used for the quality control of Rubus parvifolius L. .

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What this paper is about

Objective; To establish a method for content determination of total saponins in Rubus parvifolius L.. Methods; Total saponins was determined by UV - VIS spectrophotometry after different extracts of the sample had been coloured. Results; The method was linear in the range of 22-176μg (r = 0.9997),and the average recovery was 102. 1 % , RSDwas 2. 82% (n = 6). The content of total saponins was highest by the method of 70% ethanol reflux. Conclusion; The sample is stable after coloured. The method is sensitive and reliable , which may be used for the quality control of Rubus parvifolius L. .

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Available abstract

Objective; To establish a method for content determination of total saponins in Rubus parvifolius L.. Methods; Total saponins was determined by UV - VIS spectrophotometry after different extracts of the sample had been coloured. Results; The method was linear in the range of 22-176μg (r = 0.9997),and the average recovery was 102. 1 % , RSDwas 2. 82% (n = 6). The content of total saponins was highest by the method of 70% ethanol reflux. Conclusion; The sample is stable after coloured. The method is sensitive and reliable , which may be used for the quality control of Rubus parvifolius L. .

Key concepts: Rubus, Chemistry, Chromatography, Biology, Botany

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