The influence of short-term and long-term regular vitamin D intervention to renin-angiotensin system in spontaneously hypertensive rats

Objective To explore the influence of vitamin D intervention on renin-angiotensin system(RAS) and blood pressure in spontaneously hypertensive rats by short-term and long-term vitamin D intervention.Methods Twenty spontaneously hypertensive rats were randomly divided into short-term intervention group(S) and long-term intervention group(L),each group had 10 rats.Short-term intervention experimental group(SE,5 rats) was given vitamin D 3 μg·kg-1·d-1 dissolved in 100 μL propylene glycol by intraperitoneal injection for one week,and short-term intervention control group(SC,5 rats) was given the equal volume of propylene glycol alone.Long-term intervention experimental group(LE,5 rats) was received vitamin D preparation 3 μg·kg-1·d-1 dissolved in 100 μL propylene glycol by intraperitoneal injection(twice a week) for 12 weeks;Long-term intervention control group(LC,5 rats) was given the equal volume of propylene glycol alone.Monitored the blood pressure of rats during the experiment every week.Rats were respectively executed at 4 weeks later and 12 weeks later.Serum and organization were collected.Serum 25(OH)D3,calcium,renin,angiotensin II and aldosterone concentration were detected by enzyme-linked immunosorbent assay(ELISA).Pathological changes were examined by HE staining.Results The impact of short-term vitamin D intervention on blood pressure and RAS in spontaneously hypertensive rats was not significant.After long-term vitamin D intervention,the blood pressure of LE group was lower than that in LC group(P0.05).Serum 25(OH)D3,calcium concentration in LE group were higher than that in LC group(P0.05),Renin,angiotensin Ⅱ,aldosterone concentration were lower than those in LC group(P0.05).HE staining displayed there were hypertensive pathological changes in LC group,but which was not obvious in LE group.Conclusion Long-term vitamin D intervention could inhibit RAS,lower blood pressure,inhibit or reverse hypertensive pathological changes.