Effects of vitamin D3 on inflammation in hypertensive target organs in spontaneous hypertensive rats

Objective To explore the relationship between vitamin D3 and inflammation in hypertension.Methods Twenty 8-week-old male spontaneous hypertensive rats were randomly divided into two groups.The rats in the experimental group received vitamin D3 3 μg / kg treatment twice a week for three months,and the rats in control group were injected with propylene glycol.Blood pressure of rats were measured once a week during the experiment period.The concentrations of serum 25(OH) D 3,calcium,IL-6,and MMP-9 were detected by enzyme-linked immunosorbent before and after the experiment.The kidney / body weight ratio and heart / body weight ratio were calculated.Biopsy of the kidney,heart and artery were done for pathological examination using HE staining.Result Blood pressure between the experimental and control groups before the intervention was not significantly different(P 0.05).Three months later,the mean systolic blood pressure in the experimental group was(157 ± 9) mmHg,significantly lower than(173 ± 8) mmHg in the control group(P 0.05).25(OH) D 3 and Ca concentration were higher in the experimental group than those in the control group(P 0.05).IL-6 and MMP-9 in the experimental group were significantly lower than those in the control group(P 0.05).The heart / body weight ratio in the experimental group was significantly lower than that in the control group(P 0.05).Pathological examination showed that there were significant hypertensive and inflammatorg changes in the kidney,heart and aorta of the control group,while the experimental group was much better.Conclusions Regular administration of vitamin D3 medication can regulate the blood pressure by suppressing inflammatory factors IL-6 and MMP-9 and inhibiting inflammatory response in the hypertensive rats.