ASSA13-14-15 The Influence of Vitamin D to Inflammation of Target Organs in Spontaneously Hypertensive Rats

Objective To explore the relationship of vitamin D and inflammation of hypertension. Methods 20 spontaneously hypertensive rats were randomly divided into two groups. The experimental group received vitamin D3 preparation 3μg/kg dissolved in 0.5ml propylene glycol by intraperitoneal injection (twice a week) for three months; the control group was given the pure propylene glycol. Monitor the changes of blood pressure of rats during the experiment (once a week). Serum was collected after the experiment. Serum 25 (OH) D3, calcium, IL-6, MMP-9 concentration were detected by Enzyme-linked immunosorbent. Collect the heart and kidneys, calculate the kidney/body weight ratio and heart/body weight ratio. Kidney, heart and aorta were done the biopsy for HE staining, observation of the experimental group and control group rats pathological change. Results Blood pressure between the experimental group and control group before the intervention was no significant difference (P > 0.05). Three months later, the mean systolic blood pressure in the experimental group was (157 ± 9.1) mmHg, which was (172 ± 7.9) mmHg in control rats, difference was significant (P < 0.05). 25 (OH) D3 concentration was higher in experimental group than that in the control group (P < 0.05), IL-6, MMP-9 inthe experimental group were lower than that in the control group (P < 0.05), no significant difference with calcium between the two groups (P > 0.05). The heart/body weight ratio in the experimental group is less than that in control group (P < 0.05). Biopsy showed that there is significant hypertensive performance with the kidney, heart and aorta of the control group, while the experimental group was not obvious, and with no significant difference between normal tissues. Conclusions The law of vitamin D medication could suppress inflammatory factors IL-6 and MMP-9, inhibite the inflammatory response, and regulate the blood pressure.