Expression and significance of BAG-1 gene in precancerous lesion of breast cancer

Objective:To research the expression of anti-apoptotic gene Bag-1 in precancerous lesion of breast cancer and the expression levels of Bcl-2 and Bax,explore the correlation between Bag-1 and Bcl-2,Bax,evaluate its clinical significance.Methods:Immunohistochemical SP method was used to detect the expression levels of Bag-1,Bcl-2,and Bax in 15 specimens of breast flat epithelial atypia(FEA),20 specimens of breast ductal carcinoma in situ(DCIS),20 specimens of breast invasive ductal carcinoma(IDC),and 20 specimens of normal breast tissue;the clinicopathological data of all the patients were collected for statistical analysis.Results:The positive expression rates of Bag-1,Bcl-2,and Bax in 15 specimens of breast FEA,20 specimens of breast DCIS,20 specimens of breast IDC,and 20 specimens of normal breast tissue were 33.3%,33.3%,20.0%;70.0%,30.0%,50.0%;70.0%,50.0%,65.0%;25.0%,30.0%,10.0%,respectively.The positive expression rate of Bag-1 in breast FEA was significantly lower than those in breast IDC and breast DCIS(P0.05),the positive expression rate of Bag-1 in normal breast tissue was significantly lower than those in breast IDC and breast DCIS(P0.01).The positive expression rate of Bax in breast FEA was significantly lower than that in breast IDC(P0.01),the positive expression rate of Bax in normal breast tissue was significantly lower than those in breast IDC and breast DCIS(P0.01).There was a positive correlation between positive expression rate of Bag-1 and positive expression rate of Bcl-2 in breast FEA(P0.01).In breast DCIS,there was a positive correlation between positive expression rate of Bag-1 and positive expression rate of Bax,and there was also a positive correlation between positive expression rate of Bcl-2 and positive expression rate of Bax(P0.01).Conclusion:Bag-1 and Bcl-2 can promote each other in the course of malignant transformation from breast FEA to breast cancer,the occurrence and development of breast cancer is regulated by multiple genes,multiple factors affect the proliferation of tumor.