Expression of bcl-2,bax protein and its clinical significance in breast cancer

Objective To investigate the expression of bcl-2 and bax,to reveal the correlation between their expression and clinical-pathology parameters of breast cancer and to analyze the effects on the pathogenesis and progression of breast cancer.Methods The data of clinic and pathology with 21 cases of adenosis of breast,21 cases of fibroadenoma of breast,and 96 cases of breast caner was respectively collected.All specimens were evaluated by histopathology,immunohistochemistry technique with antibodies against of bcl-2 and bax.The correlationship of these factors was analyzed.12 cases of every disease were collected.All fresh specimens were evaluated by Western blot technique with antibodies against of bcl-2.Results The positive rate of bcl-2 protein expression in adenosis,fibroadenoma and carcinoma of breast was 95.24%(20/21)、76.19%(16/21) and 61.46%(59/96) respectively.There was significant difference between bcl-2-positive and bcl-2-negative patients in these diseases(χ2=9.790,P=0.007).The ratio of the average OD value of the bcl-2 protein expression evaluated by Western blot technique in adenosis,fibroadenoma,duct cancer in situ and invasive carcinoma of breast were 0.828、0.719、0.524 and 0.520.The result of bcl-2 protein expression was evaluated by Western blot technique bax-positive and bax-negative patients from breast carcinoma and benign lesions of breast.There was not significant difference between bax protein expression with the numbers of lymph glands and clinical stages.Conclusion Bcl-2 expression in benign lesions of breast is significantly higher than that in breast cancer and bax expression in breast cancer is significantly higher than that in benign lesions of breast.Bcl-2 expression is associated with the development and prognosisof breast carcinoma.