Study on Purification of Ampelopsis grossedentata Flavones with Macroporous Adsorption Resin and Structural Identification of Main Composition in Them

The static adsorption and desorption properties among 11 kinds of macroporous adsorption resins to Ampelopsis grossedentata flavones were compared, respectively. The results showed that resin NO.1 is the best for the purification of flavones with an adsorption capability of 41.41 mg/g and a desorption rate of 89.5%. Furthermore, the dynamic adsorption and desorption process parameters of resin NO.1 were optimized. The results indicated that when the concentration of crude flavones is 7 mg/ ml, the volume of sample is 50 to 60 ml on resin NO.1 column of 2.5 × 50 cm, the flow rate is 1.8 ml/min and the eluent is 95% ethanol, the elution rate of flavones is 90% and the purity of obtained flavones is about 65%. Finally, the above flavones product was further purified by means of recrystallization. The main composition of Ampelopsis grossedentata flavones is identified as dihydromyricetin via UV-Vis spectrum, IR spectrum, and LC-MS chromatography analysis.