Purification of total flavones from Ampelopsis grossedentata with macroporous resins

The content of flavonoids was determined by spectrophotometry. Screening the optimum conditions of purification flavonoids from Ampelopsis grossedentata by macroporous resin. The result showed: HPD100 resin is the best for the purification of flavones, when the concentration of crude flavones was 137. 8μg·mL-1,the volume of sample was 5 mL,the time of adsorption was 30 min, flow rate was 1. 5 mL / min and the eluent was 95% ethanol,the adsorption rate was 85. 44%,the elution rate of flavones was 94. 92%,the rate of recovery was 95. 66%,Under the optimum conditions the falconoid compounds quality score was from 2. 76% to 81. 10%. The experiment showed that this technology was reasonable and practicable,suitable for industrial production.