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Invitro Micropropagation of the Filament by Lilium Manissa

Yin Li

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Abstract

The filament of Lilium Manissa was used as explants for adventitious buds,roots induction and established micropropagation system. The results showed that the optimum medium for adventitious buds was MS + 0. 5 mg / L 6-BA + 0. 3 mg / L NAA,and for regeneration adventitious buds was MS + 0. 6 mg / L 6-BA +0. 6 mg / L NAA,the proliferation rate of shoot was up to 3. 76. The optimum medium for rooting was MS + 0. 1mg / L NAA,rooting rate was 100%. The plantlets were better in MS of no hormone. The plantlets were transplanted in the matrix with mixed sand,and the survival rate was 92%.

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What this paper is about

The filament of Lilium Manissa was used as explants for adventitious buds,roots induction and established micropropagation system. The results showed that the optimum medium for adventitious buds was MS + 0. 5 mg / L 6-BA + 0. 3 mg / L NAA,and for regeneration adventitious buds was MS + 0. 6 mg / L 6-BA +0. 6 mg / L NAA,the proliferation rate of shoot was up to 3. 76. The optimum medium for rooting was MS + 0. 1mg / L NAA,rooting rate was 100%. The plantlets were better in MS of no hormone. The plantlets were transplanted in the matrix with mixed sand,and the survival rate was 92%.

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Available abstract

The filament of Lilium Manissa was used as explants for adventitious buds,roots induction and established micropropagation system. The results showed that the optimum medium for adventitious buds was MS + 0. 5 mg / L 6-BA + 0. 3 mg / L NAA,and for regeneration adventitious buds was MS + 0. 6 mg / L 6-BA +0. 6 mg / L NAA,the proliferation rate of shoot was up to 3. 76. The optimum medium for rooting was MS + 0. 1mg / L NAA,rooting rate was 100%. The plantlets were better in MS of no hormone. The plantlets were transplanted in the matrix with mixed sand,and the survival rate was 92%.

Key concepts: Explant culture, Micropropagation, Lilium, Shoot, Botany, Protein filament, Murashige and Skoog medium, Biology

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