2005•Unpublished venueRequires access

Determination of ellagic acid in the extracts of Punica granatum L.pericarp by HPLC

Benhong Zhou

Abstract

Objective To develop a rapid and sensitive high-performance liquid chromatographic assay for determination of ellagic acid in the extracts of Punica granatum L.pericarp.Method Chromatography was performed on a Zobax SB-C_(18) column(250 mm×4.6 mm,5 μm)at 254 nm.The mobile phase consisted of a mixture of methanol,ethyl acetate,0.05 mol/L KH_2PO_4-H_3PO_4(34∶2∶64) at a flow rate of 1.0 ml/min.Result and Conclusion The linear range of ellagic acid was 0.3184-7.96 μg with a correlation coefficient of 0.9999.The average recovery was 98.12% and RSD 1.13%.The method is accurate and reproducible for quality control of Punica granatum L.pericarp.

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Key concepts: Punica, Ellagic acid, Chromatography, Chemistry, High-performance liquid chromatography, Correlation coefficient, Ethyl acetate, Methanol