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2011•Journal of Jinan UniversityRequires access

Determination of Ellagic acid in Polygonum chinense L. by RP-HPLC

Wen‐Cai Ye

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Abstract

Ellagic acid in Polygonum chinense L.was determined by a RP-HPLC method using s XB-C18 column(4.6 mm×250 mm,3 μm).The mobile phase consisted of methanol and 0.2% formic acid at a flow rate of 0.8 mL/min.The detection wavelength was set at 357 nm and the column temperature was 30 ℃.The results show that the linear range of ellagic acid was 0.067～2.68 μg(r=0.9999).The average recovery was 99.6 %(RSD=1.09 %),and the content of ellagic acid in ten samples was in the range of 0.592～3.439 mg·g-1.This method is found to be quick,simple and reproducible,and it is quite suitable to be used for the determination of ellagic acid in Polygonum chinense L.

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What this paper is about

Ellagic acid in Polygonum chinense L.was determined by a RP-HPLC method using s XB-C18 column(4.6 mm×250 mm,3 μm).The mobile phase consisted of methanol and 0.2% formic acid at a flow rate of 0.8 mL/min.The detection wavelength was set at 357 nm and the column temperature was 30 ℃.The results show that the linear range of ellagic acid was 0.067～2.68 μg(r=0.9999).The average recovery was 99.6 %(RSD=1.09 %),and the content of ellagic acid in ten samples was in the range of 0.592～3.439 mg·g-1.This method is found to be quick,simple and reproducible,and it is quite suitable to be used for the determination of ellagic acid in Polygonum chinense L.

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Available abstract

Ellagic acid in Polygonum chinense L.was determined by a RP-HPLC method using s XB-C18 column(4.6 mm×250 mm,3 μm).The mobile phase consisted of methanol and 0.2% formic acid at a flow rate of 0.8 mL/min.The detection wavelength was set at 357 nm and the column temperature was 30 ℃.The results show that the linear range of ellagic acid was 0.067～2.68 μg(r=0.9999).The average recovery was 99.6 %(RSD=1.09 %),and the content of ellagic acid in ten samples was in the range of 0.592～3.439 mg·g-1.This method is found to be quick,simple and reproducible,and it is quite suitable to be used for the determination of ellagic acid in Polygonum chinense L.

Key concepts: Ellagic acid, Formic acid, Chromatography, Polygonum, High-performance liquid chromatography, Chemistry, Methanol, Polyphenol

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