The Effects of Wenzhongjianpi Decoction on the Mast Cell in the Colon Mucosa of Rats with Diarrhea Irritable Bowel Syndrome

Objective:To probe the effects of Wenzhongjianpi Decoction on the mast cell(MC)in the colon mucosa of rats with diarrhea irritable bowel syndrome(IBS).Methods:Forty-five male wistar rats were randomly divided into 4 groups:normal group(NG),model group(MG),Wenzhongjianpi group(WG)and Dicetel group(DG).The diarrhea IBS model was made by Fanxieye and bondage stress stimulus.The MCs were stained by toluidine blue,the population,area,perimeter,cyclo-dimension and equivalent diameter of the MCs were determined by quantified analysis system.Results:Compared with the NG,the population,area,perimeter and equivalent diameter of the MCs were significantly increased and the cyclo-dimension was decreased in MG,WG and DG.Compared with MG,the population,area,perimeter and equivalent diameter of the MCs were significantly decreased and the cyclo-dimension was increased in WG and DG.There were no significant differences between the WG and the DG.Conclusion:The Wenzhongjianpi decoction can reduce the number of the mucosal MCs in the diarrhea IBS rats,and can inhibit the activation and degranulation of the MC,which may be one of the actions mechanisms of Chinese herbs using to treat IBS.