Optimization of Purification Technology of Total Flavonoid from Abrus cantoniensis by D101 Macroporous Resin

Objective:To optimize purification technology conditions of total flavonoid from Abrus cantoniensis by D101 macroporous resin.Method:With rutin as reference,the content of total flavonoid was determined by UV colorimetry,with adsorption rate and desorption rate as indexes,conditions of absorption and desorption were investigated by single factor test.Result:The best technology conditions were as follows:the concentration of sample solution 3 g · L-1,sample flow rate 3 BV · h-1,with 2 BV 80% ethanol as eluant,pH 6.0,elution flow rate 3.5 BV · h-1.Under these conditions,purity of total flavonoid was increased from 17.95% to 52.80%.Conclusion:D101 macroprous resin had good purification effect for total flavonoid from A.cantoniensis,optimized process was simple and feasible.