Research on extracting process of total flavonoid in mulberry seeds by orthogonal experiment

Objective To determine the optimum extracting process of total flavonoid from mulberry seeds.Methods With the extraction rate of total flavonoid from mulberry seeds as index,the influencing factors of extraction time,extraction temperature,solvent times were inspected and the optimum extracting process of total flavonoid from mulberry seeds was determined by orthogonal experiment.Results The order of factors was extraction temperature,solvent times,extraction time.The optimum extracting process of total flavonoid:extraction time 1.0 h,extraction temperature 80 ℃,12 solvent times.The extraction rate of total flavonoid from mulberry seeds was 0.24%.Conclusion This process was stable,feasible and energy saving with short production cycle and high extraction efficiency,and it was suitable for industrial production.