Optimization of the Microwave-assisted Water Extraction Process for Total Flavonoids from Mulberry Leaves

To optimize the microwave-assisted water extraction process for total flavonoids from mulberry leaves,material mass concentration,extraction time and microwave power were selected to conduct single factor tests under the condition of extraction temperature 100 ℃. Furthermore,a three-level three-variable Box-Behnken design and response surface analysis were applied using extraction rate of total flavonoids from mulberry leaves as response value to optimize the above extraction condition. The optimized parameters are composed of extraction temperature 100 ℃,material mass concentration 0. 027 7 g /mL,extracted time 4. 4 min and microwave power 377 W. Under this optimum extraction condition,the predicted extraction rate of total flavonoids from mulberry leaves was 2. 334% and the actual extraction rate was 2. 325%. The results indicated that the optimized microwave-assisted water extraction for total flavonoids from mulberry leaves has the advantages of short extraction time and high extraction yield,and is environment-friendly as well.