Determination of major components in Wenfeihuaxian formula by ultraviolet spectrophotometry

Objective To determine the contents of major components in Wenfeihuaxian formula.Methods The contents of total saponin from Wenfeihuaxian formula were determined by vanillin-ice acetic acid-perchloric method.The contents of total saponin from Wenfeihuaxian formula were determined by vanillin-ice acetic acid-perchloric method.The contents of total saponin from Wenfeihuaxian formula were determined by vanillin-ice acetic acid-perchloric method.The contents of total saponin from Wenfeihuaxian formula were determined by vanillin-ice acetic acid-perchloric method.Results The regression equation of rutin was A = 0.0026C-0.00152( r = 0.999 0).The results showed that total flavonoids of Wenfeihuaxian formula was( 7.37 ±0.76) mg·ml-1.The regression equation of gallic acid was A = 0.0095C + 0.0248( r = 0.999 5).The results showed that total polyphenols of Wenfeihuaxian formula was( 11.83 ± 0.12) mg·ml-1.The regression equation of diosgenin was A = 2.2540C +0.0544( r = 0.999 7).The results showed that total flavonoids of Wenfeihuaxian formula was( 20.75 ± 2.43) mg·ml-1.The regression equation of glucose was A = 0.0133C + 0.0463( r = 0.999 0).The results showed that total flavonoids of Wenfeihuaxian formula was( 154.81 ± 3.00) mg·ml-1.Conclusion Ultraviolet spectrophotometry is a simple and quick approach to of Wenfeihuaxian formula with accuracy and sensitivity.The results provide a scientific basis for new drug development of Wenfeihuaxian formula.