Determination of Total Flavonoids in Smilacina japonica A. Gray

[Objective] To establish a method for determining total flavonoids in Smilacina japonica A.Gray.[Method]Taking rutin as control,the total flavonoids were determined by ultraviolet-visible spectrophotometry at 505 nm,the color reagent was a mixture of NaNO2-Al(NO3)3-NaOH solution,the best color reagent formula was determined by the orthogonal experiment.[Result] The orthogonal experiment results showed that the best color reagent formula was 2.0 ml 50 mg/ml NaNO2,0.5 ml 100 mg/ml Al(NO3)3 and 15.0 ml 40 mg/ml NaOH.There was a good linear relationship between the total flavonoids content and absorbance in the concentration range of 0.004-0.028 mg/ml,R2=0.999 6.Among the different parts,the contents of total flavonoids in the stem leaf was higher,reached 1.8%;in the rhizome was lower,only 0.43%;the lowest in the seed.The contents of total flavonoids in the rhizome of Smilacina japonica at the middle ten days of September was the highest among the different harvesting time.[Conclusion] The method was simple,fast and accurate,and it had been applied to determine flavonoids in Smilacina japonica successfully;these primary research results supplied credible foundation for quality estimate and utilization of Smilacina japonica A.Gray.