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2009•CHINESE JOURNAL OF ANALYTICAL CHEMISTRY (CHINESE VERSION)Requires access

Separation and Purification of Lentinan by Preparative High Speed Counter Current Chromatography

Zhi Jiang

Open publisher page 6 citations

Abstract

High speed counter current chromatography(HSCCC) had been applied for the separation of lentinan.The HSCCC run was carried out with a two-phase solvent system composed of polyethylene glycol 1000(PEG1000)-dipotassium hydrogen phosphate-monopotassium phosphate-water(0.5:1.25:1.25:7.0,m/m) by eluting the lower aqueous phase at 1.5 mL/min at 500 r/min.Under the optimized conditions,One-step HSCCC procedure yielded 95 mg of LenⅠ and 45 mg of LenⅡ.By Sephadex G-100 column chromatography,LenⅡ was identified to be homogenous,and the molecular weight was 293 kDa according to the method of gel-filtration.The IR sepectrum of LenⅡ showed polysaccharide a character.

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What this paper is about

High speed counter current chromatography(HSCCC) had been applied for the separation of lentinan.The HSCCC run was carried out with a two-phase solvent system composed of polyethylene glycol 1000(PEG1000)-dipotassium hydrogen phosphate-monopotassium phosphate-water(0.5:1.25:1.25:7.0,m/m) by eluting the lower aqueous phase at 1.5 mL/min at 500 r/min.Under the optimized conditions,One-step HSCCC procedure yielded 95 mg of LenⅠ and 45 mg of LenⅡ.By Sephadex G-100 column chromatography,LenⅡ was identified to be homogenous,and the molecular weight was 293 kDa according to the method of gel-filtration.The IR sepectrum of LenⅡ showed polysaccharide a character.

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Available abstract

High speed counter current chromatography(HSCCC) had been applied for the separation of lentinan.The HSCCC run was carried out with a two-phase solvent system composed of polyethylene glycol 1000(PEG1000)-dipotassium hydrogen phosphate-monopotassium phosphate-water(0.5:1.25:1.25:7.0,m/m) by eluting the lower aqueous phase at 1.5 mL/min at 500 r/min.Under the optimized conditions,One-step HSCCC procedure yielded 95 mg of LenⅠ and 45 mg of LenⅡ.By Sephadex G-100 column chromatography,LenⅡ was identified to be homogenous,and the molecular weight was 293 kDa according to the method of gel-filtration.The IR sepectrum of LenⅡ showed polysaccharide a character.

Key concepts: Chemistry, Chromatography, Lentinan, Countercurrent chromatography, Size-exclusion chromatography, Sephadex, Polyethylene glycol, Aqueous solution

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