Preparative isolation and purification of endormophin-1 by high-speed counter-current chromatography

Endormophin-1 synthesized by chem-biological method was purified by high-speed counter-current chromatograph technology(HSCCC).The optimum separation conditions were as follows:A two-phase solvent system was ethyl acetate-methanol-water(3∶1.5∶3,v/v/v).The lower phase as the mobile phase was operated at a flow rate of 1.0 mL /min,while the apparatus was rotated at 900 r/min,and detection were length was at 280 nm.Each time 120 mg of the crude sample was loaded under these conditions,and 71 mg of endorphin-1 with the purity of 99.1% was obtained.The recovery was 91.5%.The method was relatively simple,fast,and applicable to the large-scale isolation and separation of endormophin-1.