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Separation and Purification of Monascus Pigments by High-speed Counter-current Chromatography

Guo Yang-hao

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Abstract

Six azaphilone-type pigments were separated from Monascus-fermented solid medium and purified/fractionated by by high-speed counter-current chromatography (HSCCC). A solvent system with weak polarity involving four components nhexane,ethyl acetate,methanol and water was selected and used for HSCCC. Based on a comparative analysis of partition coefficients of target compounds in different solvent systems,a two-step HSCCC routine was developed. After separation by HSCCC and crystallization with acetone,six azaphilone-type pigments with high purity were obtained. The purity of each pigment was above 98.5% and their yields were between 81.40% and 84.78%. Their molar absorption coefficients were 13313,13877,9380,9360,25621 L/(mol·cm) and 25849 L/(mol·cm),separately.

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What this paper is about

Six azaphilone-type pigments were separated from Monascus-fermented solid medium and purified/fractionated by by high-speed counter-current chromatography (HSCCC). A solvent system with weak polarity involving four components nhexane,ethyl acetate,methanol and water was selected and used for HSCCC. Based on a comparative analysis of partition coefficients of target compounds in different solvent systems,a two-step HSCCC routine was developed. After separation by HSCCC and crystallization with acetone,six azaphilone-type pigments with high purity were obtained. The purity of each pigment was above 98.5% and their yields were between 81.40% and 84.78%. Their molar absorption coefficients were 13313,13877,9380,9360,25621 L/(mol·cm) and 25849 L/(mol·cm),separately.

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Available abstract

Six azaphilone-type pigments were separated from Monascus-fermented solid medium and purified/fractionated by by high-speed counter-current chromatography (HSCCC). A solvent system with weak polarity involving four components nhexane,ethyl acetate,methanol and water was selected and used for HSCCC. Based on a comparative analysis of partition coefficients of target compounds in different solvent systems,a two-step HSCCC routine was developed. After separation by HSCCC and crystallization with acetone,six azaphilone-type pigments with high purity were obtained. The purity of each pigment was above 98.5% and their yields were between 81.40% and 84.78%. Their molar absorption coefficients were 13313,13877,9380,9360,25621 L/(mol·cm) and 25849 L/(mol·cm),separately.

Key concepts: Countercurrent chromatography, Chromatography, Monascus, Pigment, Chemistry, Solvent, Acetone, Ethyl acetate

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Separation and Purification of Monascus Pigments by High-speed Counter-current Chromatography — Research Paper | ScholarLens